Effects of Intermittent Irradiation with Low-Level LED Light on Osteoblast-Like Cells Derived from Rat Bone Marrow

Recently, low level laser therapy (LLLT) has been reported to promote wound healing of fractures, tooth extraction sockets and bone-dental implants osseointegration. In this study, we investigated the effects LLLT on cell proliferation, differentiation calcification rat bone marrow-derived osteoblast-like cells using blue LED light irradiation at a wavelength 455 nm. The conditions were as follows; with nm, 510 mW output power (measured value in irradiated field: 100 mW/cm2), distance: 20.8 mm, for 14 seconds every 1.5 hours an energy 5.6 joules/cm2 (up 4.5 hours). experimental groups divided into 4 groups; control group, light-irradiation FGF-containing media group (n=3). proliferative ability was determined Cell Counting Kit-8 immediately after first irradiation, 1.5, 3.0, 12 by ELISA. Lab assay ALP used search potential, activity levels samples 3 7 days. And staining also performed days, positive analyzing percentage stained area each well. For cellular activity, Alizarin Red determine its positivity rate fraction well 28 all searched results, both proliferation abilities highest group. significantly higher LED, FGF LED▪FGF than that results study suggested useful tissue regeneration.